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KMID : 1123920160300030150
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Korean Journal of Oriental Physiology and Pathology
2016 Volume.30 No. 3 p.150 ~ p.156
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Extract of Broussometia kazinoki Induces Apoptosis Through the Mitochondria/Caspase Pathway in A549 Lung Cancer Cells
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Kim Tae-Hyun
Kim Dan-Hee
Mun Yeun-Ja
Lim Kyu-Sang
Woo Won-Hong
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Abstract
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Extract of Broussometia kazinoki Rhizodermatis has been traditionally used for geopoong, diuresis, hwalhyeol. In the present study, the apoptotic effect of methanol extract of Broussometia kazinoki (MBK) were investigated. Cell viability of A549 cells was measured by MTT assay. Apoptosis-related protein and MAPK protein levels were measured by Western blot. Chromatin condensation of A549 cells was stained with DAPI. MBK inhibited cell proliferation of A549 cell. Based on DAPI staining, MBK-treated cells manifested nuclear shrinkage, condensation and fragmentation. Treatment of A549 cells with MBK resulted in activation of the caspase-3, -8, -9 and cleavage of poly ADP-ribose polymerase (PARP). In the upstream, MBK increased the expressions Bax and Bak, decreased the expression of Bcl-2, and augmented the Bax/Bcl-2 ratio. MBK-induced apoptosis was accompanied by sustained phosphorylation of JNK, p38 MAPK and apoptosis signal-regulating kinase (ASK)-1. These results suggest that MBK induced apoptosis in A549 cells through Bcl-2 family protein-mediated mitochondria/caspase-3 dependent pathway. In addition, MBK increased the activation of ASK-1, which are critical upsteam signals for JNK/p38 MAPK activation in A549 cancer cells.
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KEYWORD
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Broussometia kazinoki, Apoptosis, Bcl-2 family, MAPK, ASK-1
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